What Are the Characteristics of Solid Phase Extraction?

Solid phase extraction is essentially a liquid chromatographic separation. The main separation mode is the same as that of liquid chromatography. It can be divided into normal phase (the polarity of the adsorbent is greater than the polarity of the eluent), and the reverse phase (the polarity of the adsorbent is less than Eluent polarity), ion exchange and adsorption.

The adsorbent used for solid phase extraction is also the same as the stationary phase commonly used in liquid chromatography, except that it differs in particle size.

SPE uses a solid adsorbent to adsorb a target compound in a liquid sample, separates it from the matrix and interfering compound of the sample, and then elutes with an eluent or desorbs by heating to achieve separation and enrichment of the target compound.

Because of its safety, high recovery rate, good reproducibility, easy operation, fast, wide application range, easy to achieve automatic operation. Hawach offers you solid phase extraction cartridges.

Operation Instruction of Three Different SPE Cartridges

The solid-phase extraction (SPE) devices are extremely popular equipment widely used in the sample pre-treatment technology. Among them, the SPE cartridge plays a significant role in the sample pretreatment process. Here are three different kinds of SPE cartridges below that could meet the various demands of the customers.

Instruction of Reversed-phase SPE Cartridge

The reversed-phase (RP) SPE cartridge is used to extract non-polar samples from polar matrices.

The first step is activation – rinse the cartridge with 3-5 ml of methanol or deionized water, which could keep the cartridge moist. Next, the sample needs to be evenly passed through the cartridge at a suitable flow rate. The third step is cleaning, which indicates the cartridge with the adsorbed sample has to be eluted with 5 ml of a polar solvent. (commonly used pure water) the last step is to elute the sample in batches with 1-5 ml of non-polar solvent to the collection container.

Instruction of Normal-phase SPE Cartridge

The normal-phase (NP) SPE cartridge is suitable for extracting polar samples from non-polar matrices. The first step is to rinse the cartridge with 3-5 ml of non-polar solvent. The next steps are just like using the RP SPE cartridge but only to clean the cartridge with a non-polar solvent and to elute the sample with a polar solvent.

Instruction of Ion-exchange SPE Cartridge

The ion exchange SPE cartridge is available for extracting charged samples. The cartridge is rinsed with 3-5 ml of deionized water or low ionic strength buffer. It uses a 5 ml deionized water / or low ionic strength buffer to clean the cartridge itself and elute the sample with 1-5 ml of high ionic strength buffer.