The Operation Methods and the Selection of SPE Columns
The quality of the HAWACH SPE cartridge is stable and reliable and is ideal for different volume sample processing. SPE cartridges can be customized according to the customer’s requirements. It is easy to use, fast and efficient, with multiple stationary phase options. Moreover, for routine analysis, they are attractively priced for high-throughput laboratories, which is solvent saving and have high sample recovery.
Operation Methods of the reverse phase extraction cartridge
Extract non-polar samples from polar matrices
1. Activation: rinse the extraction column with 3-5 ml of methanol; rinse it with 3-5 ml of deionized water (or buffer) and keep it moist.
2. Loading: The sample is evenly passed through the extraction column at a suitable flow rate.
3. Cleaning: The extraction column of the adsorbed sample is eluted with 5ml of the solvent of appropriate polarity (pure water, buffer, and water/organic solvent mixture are commonly used).
4. Elution: The sample to be tested is eluted in batches with 1-5ml of non-polar solvent to the collection container.
Such as reversed phase C8 SPE cartridges, reversed phase C18 SPE cartridges, and reversed phase C18A SPE cartridges.
Operation method of Normal phase extraction cartridge
Extracting polar samples from non-polar matrices
1. Activation: 3-5 ml non-polar solvent elution extraction column
2. Cleaning: The extraction column of the adsorbed sample is eluted with 5 ml of a suitable non-polar solvent.
3. Elution: The sample to be tested is eluted in batches with 1-5 ml of a polar solvent in the collection container.
Such as normal phase Silica SPE cartridges, Normal Phase PR Grade Florisil SPE Cartridges, and Normal Phase Diol SPE Cartridges.
Operation method of Ion exchange SPE cartridge
Extracting charged samples
1. Activation: Rinse the extraction column with 3-5 ml of deionized water/or low ionic strength buffer.
2. loading: at a lower flow rate, the sample is evenly passed through the extraction column
3. Cleaning: 5 ml deionized water / or low ionic strength buffer rinse extraction column
4. Elution: The sample to be tested is eluted into the collection container with 1-5 ml of high ionic strength buffer.
Such as Ion-Exchange SCX SPE cartridges, Ion-Exchange PRS SPE cartridges, and Ion-Exchange PRS SPE cartridges.
Wide applications of SPE cartridges
HAWACH SPE cartridges can be applied to many different industries, like
Separation and analysis of compounds in organic synthesis
Surfactants and other industrial fields
Environmental testing of water samples (drinking water, wastewater)
Detection of pesticide residues in vegetables
Analysis of seasoning aroma in food and beverage
Detection of gaseous sulfides and drug residual solvents (VOCs)
Forensic analysis of fire or explosive samples
Toxic analysis; analysis of drugs and drugs in blood and urine fluids, etc.
Selection of SPE column
1: Graphitized carbon and composite (for pesticide residues)
2: Polymer matrix (HLB-MCX-MAX-WCX-WAX)
3: Silica matrix normal phase (silica gel-fluorosilicate-alumina-Diol-PSA-amino)
4: Silica matrix ion exchange (SAX-SCX-PRS)
5: Silica matrix reversed-phase (C18-C8-CN)
6: Pretreatment and analytical applications (solid-phase extraction)
7: C8-SCX-SAX mixed series (drug metabolism and abuse)
Choice of solid phase extraction cartridge
Based on chromatographic adsorbents, the solid phase extraction cartridge can be roughly divided into three categories, which are based on silica gel, polymers, and inorganic materials.
Firstly, we will discuss the first one based on silica gel. The silica gel has a strong polarity and is weakly acidic. It can be used in two separation modes: normal phase or reverse phase: normal phase extraction. It has a good extraction effect on steroids and is usually used for the extraction of non-polar or weakly polar compounds or the removal of polar impurities. The common usages include medicine, blood samples, polypeptide desalination, organic acids in beverages and trace organic compound enrichment in environmental samples, and so on.
The second type is based on high polymers, such as polystyrene-divinylbenzene, and so on. It has a high sample load, excellent resistance to polar compounds, and good retention capacity, as it is packed with high-purity, high-crosslinking styrene-divinylbenzene polymer as the stationary phase. It is usually used to retain hydrophobic compounds containing hydrophilic groups under reversed conditions, such as phenols, nitroaromatics, nitroamines, nitrates, etc.
The third type is based on inorganic materials, for instance, graphitized carbon, Flori diatomaceous earth, alumina, and so on. Florisil is a polar silica gel adsorbent compounded with magnesium oxide and its extraction cartridge can be used to adsorb polar compounds from the non-polar matrix; Graphitized carbon black (CARB) extraction cartridge has a very fast extraction process with graphitized carbon black as the filler. And the adsorption capacity for compounds is more than double that of silica gel. It is very suitable for the extraction and purification of many organic substances and is especially suitable for separation.
Customer service
Customer service is our highest priority. A person, not a phone system, will answer your call. All inquiries are responded to promptly. We offer a warranty on all parts and labor. If there is anything wrong with the cartridges or any problem with the experiment, we are here to solve it.
The product series provided by HAWACH include liquid chromatography cartridges, gas chromatography cartridges with complete fillers and complete specifications from analysis to the preparation involved in various food testing and drug testing, and sample pretreatment with complete varieties and specifications; chromatographic cartridges for detection, complete set of equipment for purification system and various fillers; at the same time, HAWACH also provides consumables of various conventional laboratory consumables, such as syringe filters, filter membranes (including sterile membranes), sample vials and pipettes and various analysis and testing instruments.
The products provided by HAWACH includes a wide range of well-prepared, well-packed liquid chromatography columns, gas chromatography columns, purified packing, and a full range of pre-treatment solid-phase extraction columns for analysis and preparation of foods and pharmaceuticals, immunoaffinity column; column for detection and system equipment for purification and various packings. Also, HAWACH provides routine laboratory consumables (such as syringe filters (including sterilizers), filter membranes, vials, and various analytical instruments.
If you need the details of the SPE column described above, as well as the order and price. Welcome to call us +86-29-89284429 or send an email to info@hawach.com.
