Summary of QuEChERs Method

AOAC.2007 method: 6g MgSO4, 1.5g sodium acetate
STEP1: Weigh 15g of low-temperature homogenized sample into 50ml capped centrifuge tube, add 15ml of 1% acetic acid acetonitrile and 100μl of internal standard solution, and shake vigorously for 1min.
STEP2: 6 g of magnesium sulfate and 1.5 g of sodium acetate (AOAC 2007.01 extraction tube packing) were added, violently shaken for 1 min, and centrifuged at 4000 r/min for 5 min.
STEP3: AOAC2007.01 purification tube (50 mg PSA + 150 mg MgSO4) or (50 mg C18/50 mg Carb), 1 mL of the supernatant was added thereto, shaken vigorously for 30 s, and centrifuged at 4000 r/min for 5 min.

EN15662-2008 Method: 4g MgSO4, 1g sodium chloride, 1g sodium citrate, 0.5g disodium hydrogen citrate
STEP1: Weigh 10g of low-temperature homogenized sample into 50ml capped centrifuge tube, add 10ml acetonitrile and 100μl internal standard solution, and shake vigorously for 1min.
STEP2: 4 g of magnesium sulfate, 1 g of NaCl, 0.5 disodium hydrogen citrate, 1 g of sodium citrate (EN15662 extraction filler) were added, violently shaken for 1 min, and centrifuged at 4000 r/min for 5 min.
STEP3: EN15662 purification tube (25 mg PSA + 150 mg MgSO4) or (25 mg C18/7.5 mg Carb), 1 mL of the supernatant was added thereto, shaken vigorously for 30 s, and centrifuged at 4000 r/min for 5 min.

NY/T 1380-2007 Method: 6g MgSO4, 1.5g sodium acetate
STEP1: Weigh 15g of homogenized sample into 50ml capped centrifuge tube, add 15ml of 1% acetic acid acetonitrile solution, and shake vigorously for 1min.
STEP2: 6 g of magnesium sulfate and 1.5 g of sodium acetate (AOAC 2007.01 extraction tube packing) were added, violently shaken for 1 min, and centrifuged at 5000 r/min for 1 min.
STEP3: 0.1 g PSA + 0.1 g C18 + 0.3 g magnesium sulfate, 2 mL of the supernatant was added thereto, vigorously shaken for 1 min, and centrifuged at 5000 r/min for 1 min. STEP4: Pipette 1 mL of the supernatant into a 1 mL volumetric flask, nitrogen to <0.8 mL, accurately add 100 μL of internal standard and 100 μL of protective agent, and make up to 1 mL of acetonitrile.
Protective agent: 3-ethoxy 1,2-dipropylene glycol; sorbitol
Internal standard: Triphenyl phosphate (TPP)．