Solid Phase Extraction Device and Basic Operation Steps

1. Solid phase extraction column

(1) SPE column

About solid phase extraction cartridge:

a. The common solid phase extraction column is divided into three parts: medical polypropylene column tube, porous polypropylene sieve plate (20μm) and filler (mostly 40-60μm, 80-100μm)

b. Common specifications: 100mg/1ml, 200mg/3ml, 500mg/3ml, 1g/6ml, etc. Take 100mg/1ml as an example, 100mg

For the mass of the packing, 1 ml is the volume of the empty column tube.

c. One-time use: In order to avoid cross-contamination and ensure the reliability of detection, SPE cartridges are usually used at one time.

2. Solid phase extraction device

(1) Vacuum SPE device composition: glass cylinder, vacuum pressure gauge, vacuum pump, vacuum buffer bottle, collection pipe rack, small column connector, large volume sampler, 12- or 24-position anti-interference device.

a. Standard StopCock valve, precise control of flow rate

b. The height of the test tube rack is adjustable to meet the needs of different volumes

c. Pressure gauge venting valve side design, easy to use

d. Compact design, durable, cost-effective

3. Basic operation steps

The operation is slightly different for the difference in the retention mechanism of the filler (the filler retains the target compound or retains impurities).

1) Filler retains target compound

Solid phase extraction operations generally have four steps:

Activation – Removes impurities from the column and creates a solvent environment.

Loading – The sample is dissolved in a solvent, transferred to the column and the components are retained on the column.

Rinse – Removes interference to a large extent.

Elution – The test substance is eluted and collected with a small volume of solvent.

2) Filler retains impurities

There are generally three steps in the operation of a solid phase extractor:

Activation – Removes impurities from the column and creates a solvent environment.

Loading – Transfer the sample into the column where most of the target compound will flow out with the sample base and the impurities will remain on the column. Therefore, this step begins to collect.

Elution – The components are rinsed off with a small volume of solvent and collected. Combine the collection solution.