Sample Pretreatment Principle, Type and Packing Selection of SPE Cartridge

Sample Pretreatment Principle of SPE cartridge

Compared with traditional liquid-liquid extraction methods, SPE can greatly improve the recovery rate of analytes, separate analytes from interferences more effectively, and reduce sample pretreatment processes. Simple, time-saving, and labor-saving, it can be widely used in medicine, food, environment, commodity inspection, chemical industry, and other fields. SPE uses solid adsorbents to adsorb the target compound in the liquid sample, separate it from the sample matrix and interfering compounds, and then use the eluent to elute or heat to desorb the target compound to achieve the purpose of separation and abdominal muscle target compound.

C8 SAX SPE Cartridges C8 SPE Columns Mixed Mode SPE Cartridge

Type of SPE Cartridge

As effective experimental equipment such as sample pretreatment and condition testing, SPE cartridge have been widely used. There are many types of solid phase extraction cartridges with different specifications. The column body of the solid phase extraction column should be high purity and smooth, no dissolution when using organic solvents, no impurities on the sieve, suitable sieve pore size, and uniform pore distribution. Silica-based ionic fillers can be divided into strong cationic fillers, strong anionic fillers, weak cationic fillers, weak anionic fillers, etc. Since ionic cartridges are mostly used under alkaline conditions, and silica gel has poor alkali resistance, ionic fillers are mostly synthesized from organic matrix baseballs.

Packing Selection of SPE Cartridge

Solid phase extraction is a process of adsorption, desorption/elution of the target substance on the stationary phase, so the factors that affect the adsorption, desorption/elution will directly affect the extraction efficiency, such as the type of filler, the strength of the elution solvent, and the pH And flow rate, etc. Packing is the core of solid-phase extraction technology. Choosing SPE cartridges packing with moderate adsorption to the target is a prerequisite to ensure accurate detection. Of course, we can choose different packings for the same target, but we must pay attention to the adjustment of the method.

Comparative advantages of SPE cartridge

Sample pretreatment is a key link in the entire analysis process. Traditional sample pretreatment techniques, such as liquid phase extraction, distillation, and filtration, are generally cumbersome and time-consuming, requiring a large number of organic solvents that are harmful to the environment and the human body and difficult to automate. Therefore, it has become a hot spot in the field of analytical chemistry to establish a new sample pretreatment technology with time-saving, high efficiency, and low consumption of organic solvents. In order to realize the automation of sample removal and minimize the use of organic solvents, Hawach’s SPE can solve the above problems.