Reversed Phase C18 SPE Cartridges

Hawach C18 SPE cartridge is a sample pre-treatment device mainly used for extraction, separation, and concentration. It is mainly applied in the pre-treatment process of the compound contained in food, agricultural and livestock products, and biological products.

Operation Principle

The solid-phase extraction technique is a separation and purification process of samples by adopting the selective adsorption and elution method, which is based on the liquid-solid phase chromatography theory.

Commonly, the sample solution needs to pass through the adsorbent first during the use of the C18 SPE cartridge. In this way, the substance under test could be retained. Secondly, wash out the impurity with a moderately strong solvent and elute the sample quickly with a small amount of solvent. Thereby, rapid separation and purification could be achieved.

Reversed phase C18 SPE cartridges are reversed phase separation that includes a sample matrix (mobile phase) of polar or moderate polarity and a non-polar solid phase. The affinity of adsorbents for solutes is mainly due to their hydrophobicity.

Packing of reversed phase C18 SPE cartridges

Octane and cation exchange resin

Reversed phase C18 SPE cartridges have an important position in solid-phase extraction. Solid phase extraction allows impurities to be retained, detecting compounds to pass through. The principle and application of the solid phase extraction column of the silica gel matrix (C18) are completely similar to that of the solid phase extraction column (C8) of the same silica gel matrix.

The force of reversed phase C18 SPE cartridges

Van der Waals force and hydrophobic force
Eluent of reversed phase C18 SPE cartridges
Organic reagents such as acetonitrile, ethyl acetate, and methanol.

Applicable scope of reversed phase C18 SPE cartridges

The C18 SPE cartridge is quite suitable for water analysis, especially in the pre-treatment analysis of the pesticide residue, organic substances, and phenolic compounds contained in water. In the analysis of residual pesticides, methanol is often used as an eluent.

As for the determination of organic substance, it requires immediate analysis as long as it is sampled to the vial due to its instability. For the analysis of phenolic compounds, it employs tetrahydrofuran as an eluent.

Suitable for non-polar to medium polar compounds, such as antibiotics, barbiturates, phthalein, caffeine, drugs, dyes, aromatic oil, fat-soluble vitamins, fungicides, herbicides, pesticides, carbohydrates, and p-hydroxytoluene substituted ester, phenol, phthalic acid ester, steroids, surfactant, theophylline, water-soluble vitamins.