Reversed phase C18 SPE cartridges are reversed phase separation that includes a sample matrix (mobile phase) of polar or moderate polarity and a non-polar solid phase. The affinity of adsorbents for solutes is mainly due to their hydrophobicity.
Applicable scope of reversed phase C18 SPE cartridges
Suitable for non-polar to medium polar compounds, such as antibiotics, barbiturates, phthalein, caffeine, drugs, dyes, aromatic oil, fat-soluble vitamins, fungicides, herbicides, pesticides, carbohydrates, and p-hydroxytoluene substituted ester, phenol, phthalic acid ester, steroids, surfactant, theophylline, water-soluble vitamins.
Packing of reversed phase C18 SPE cartridges
Octane and cation exchange resin
Reversed phase C18 SPE cartridges have an important position in the solid phase extraction. Solid phase extraction allows impurities to be retained, detecting compounds to pass through. The principle and application of the solid phase extraction column of silica gel matrix (C18) are completely similar to that of the solid phase extraction column (C8) of the same silica gel matrix.
The force of reversed phase C18 SPE cartridges Van der Waals force and hydrophobic force Eluent of reversed phase C18 SPE cartridges Organic reagents such as acetonitrile, ethyl acetate, and methanol.