Product Highlights and Tests of SPE

Product Highlights

Generally, SPE products are focused more on impurity dissolution and adsorbent. For its low impurity dissolution ability, due to all medical grade materials of PP and PE, SPE features resistance to corrosion and low content of impurities.

In addition, SPE’s superior purification function totally attributed to imported adsorbents.

Solid Phase Extraction (SPE) is a sample preparation technique used to isolate, pre-concentrate, and purify analytes from complex sample matrices. Here are some product highlights and common tests associated with SPE:

  1. Sorbent Materials: SPE cartridges are available with a variety of sorbent materials, including silica, C18 (octadecyl), ion-exchange resins, and more. These materials allow for selective retention of analytes based on their chemical properties.

Typical Application Analysis

Product Highlights and Tests of SPE

* HLB and C18 are universal columns so that all kinds of projects can import them.
* MCX, Florisil, and C18 have the common specification of 6ml.
* HLB and C18 provide the regular model of 60mg/3ml.

  1. Particle Size and Pore Size: SPE sorbents are available with different particle sizes and pore sizes, allowing for fine-tuning of the extraction process based on the size and polarity of the analytes.
  2. Cartridge Sizes: SPE cartridges come in different sizes to accommodate different sample volumes and flow rates. This allows for scalability in sample preparation.
  3. Compatibility: SPE cartridges are compatible with various solvents, making them suitable for different sample matrices and analytical methods.

Common Tests Associated with SPE:

  1. Conditioning Test:
    • Before use, SPE cartridges need to be properly conditioned with solvents to activate and stabilize the sorbent. A conditioning test checks if the cartridge is properly prepared for sample extraction.
  2. Sample Loading Efficiency:
    • This test evaluates how efficiently the sorbent captures analytes from the sample. It helps ensure that the maximum amount of analyte is retained.
  3. Washing Efficiency:
    • After sample loading, a washing step removes unwanted matrix components. This test assesses the effectiveness of the washing step in removing interfering substances.
  4. Elution Efficiency:
    • Elution is the step where analytes are recovered from the sorbent for subsequent analysis. This test checks the efficiency of the elution process in extracting analytes.
  5. Recovery and Reproducibility:
    • These tests evaluate the overall recovery of analytes and the consistency of results across multiple extractions. It assesses the repeatability and reliability of the SPE method.
  6. Matrix Effects:
    • Matrix effects occur when components in the sample matrix interfere with the analysis. This test assesses the potential for matrix effects and the effectiveness of SPE in minimizing them.
  7. Analyte Stability:
    • It’s important to verify that the analytes of interest remain stable during the entire SPE process, including sample loading, washing, and elution steps.
  8. Quality Control (QC) Samples:
    • QC samples are used to monitor the performance of the SPE method over time. They serve as a reference to ensure consistent and accurate results.

By conducting these tests, laboratories can verify the performance of their SPE method, troubleshoot any issues, and ensure reliable and reproducible results in subsequent analyses.