Principles for Selecting SPE Cartridges Specifications
Common solid phase extraction columns are mostly polyethylene-based injection syringes. The device is equipped with two pieces of polypropylene or glass fiber. The two pieces are filled with a certain amount of chromatographic adsorbent. filler). The upper end of the solid phase extraction column is open, and the lower end is a liquid outlet, and the liquid is discharged from the outlet after passing through the adsorbent. According to the volume above the solid phase extraction column packing, the specifications of the solid phase extraction column can be divided into 1 mL, 3 mL, 6 mL, 10 mL, 15 mL, 20 mL, 30 mL, 60 mL, and so on. There are also special-sized solid phase extraction columns that are produced according to specific application requirements. From the outside of the solid phase extraction column, the classic solid phase extraction column is similar to the injection syringe and is straight. Another type of solid phase extraction cartridge is funnel-shaped at the top, making it easier to load more samples at once in manual operation.
The non-sieve SPE column is an integrated new SPE column. The product uses the exclusive technology of mystery technology to specially process the bonded silica fillers such as C18 and C8 to make it have high mechanical strength and toughness. It is not necessary to add polyethylene porous baffle as support in the column. The mystery proprietary technology product “Integrated SPE Solid Phase Extraction Column [1]” is the first integrated SPE column on the domestic and international markets.
After selecting the type of SPE cartridges, we have to determine the specifications of the SPE cartridge.
The principle of selecting the SPE cartridge specification is to first ensure that SPE cartridge packing is capable enough to retain the target compound 100% on the column And the filler, on the other hand, should not be excessive. The more filler, the greater the amount of solvent that is effective for the target compound to elute. Therefore, the SPE cartridge capacity must be considered when selecting the specifications for the SPE cartridge. Cartridge capacity refers to the mass of a compound that retains a compound with a primary force per unit mass. For instance, the capacity of the bonded silica cartridge generally ranges from 1% to 5%.
Take the SPE cartridges with 100 mg filler as an example. The maximum capacity is generally less than 5 mg. The cartridge capacity of the ion exchanger is about 0.5 to 1.5 meq/g. It is worth pointing out that because we are mostly a mixture, there are many interfering substances besides the target compound. Therefore, when selecting the cartridge size, it is necessary to consider the target compound and the total amount of interference substances that may be adsorbed under the extraction conditions, and not only the target compound. Otherwise, even if the target compound does not exceed the cartridge capacity, the SPE cartridges retains some of the interfering substances while retaining the target compound.
Since the total amount of the two has exceeded the cartridge capacity, the result is that some of the target compounds cannot be retained. On the SPE cartridge. Therefore, for safety reasons, it is recommended that the cartridge capacity be better than twice the estimated target compound content.
Product advantages
1, no sieve plate, perfect to avoid the “non-specific adsorption” caused by the sieve plate.
2, the filler is stable and can be good, there will be no problems such as loose packing.
3. The integrated design of the whole packing effectively avoids the “ditch effect”.
4. It can choose packing with particle size as low as 8-10μ, which greatly improves the separation efficiency and sample loading.
You also can choose Reversed Phase C8 SPE Cartridges, Normal Phase Neutral Alumina (ALN) SPE Cartridge, Ion-Exchange PRS SPE Cartridges, Normal Phase PR Grade Florisil SPE Cartridges and other SPE Cartridges using.
