Introduction of SPE Column from Hawach
An SPE cartridge, another name is a SPE column. SPE is solid phase extraction, which is a kind of separation technique combining the processes of selective retention and selective elution and so on.
Porous polypropylene sieve plate, medical polypropylene column tube and packing are three parts of commonly used SPE cartridge. The cartridge tube is the carrier of the adsorbent, made of serum-grade polypropylene. The sieve plate acts as a fixer for adsorbent and a filter for solution.
In terms of the filler type, SPE column can be divided into four applications of silicone bonding matrix, polymer matrix, absorption matrix and specialized columns. Besides, the silicone bonding has four sorts—antiphase(C18 sealing end/uncapped/hydrophile, C8), positive phase(Silica,Diol, CN), ion exchange(SAX, SCX, NH2, PRS, PSA) and mixed mode(C8/SCX, C8/SAX). Similarly, according to HLB(Hydrophile-Lipophile Balance), the polymer one possesses series of MCX, MAX, WCX and WAX.
Alumina, graphitized carbon black and Florisil pesticide residues are involved into the absorption matrix.
The classification of an SPE cartridge:
Normal phase: Silica, NH2, CN, Diol, Florisil, Alumina, CN
Reverse Phase: C18, C8, C18A
Ion exchange: SCX, SAX, COOH, NH2
Mixed mode: PCX, PAX, C8/SCX
General process of an SPE cartridge
Activation: A suitable solvent is added to unfurl the functional groups and remove the possible distractor.
Equilibrium: Create suitable solvent environment.
Retention: The process of fixing a compound by an adsorbent.
Washing: Maximum possible removal of the distractor without affecting the retention of the target compound.
Elution: Let the solvent with stronger eluting ability pass through the adsorbent.
The acting force of an SPE cartridge includes non-polar interaction, polar interaction, ion interaction and secondary interaction.
|Material||Any part of the material directing touched by terminal customers would contaminate their samples, so there are instructions set on every material section.|
|Parameters of Stationary Phase||matrix type (e.g. silica gel or polymer)|
bonded phase type (e.g. C18 or CN)
particle size (e.g. 3or 5 um)
surface area of stationary phase (e.g. 130 m2/g)
purity of stationary phase
|Column Specification||It means the volume or the filling content, which decides the Sample throughput.|
It consists of item number, specification and package(pcs/pk).
For instance, SLSPE1001SI stand for SPE column from Hawach with 100 mg and made of Silica.