How To Operate When The SPE Cartridge Activates The Adsorbent?
As we know, five steps required for solid phase extraction cartridge: sample preparation, activation or equilibration of the column, loading, rinsing and eluting the compound of interest. There is no uniform regulation for that the solid phase extraction cartridge should be activated, rinsed and eluted with what kind of solvent. Please refer to the national standard, pharmacopeia or proven method based on your analysis.
There are two purposes for the activation of the solid phase extraction cartridge. The first purpose is to infiltrate the packing so that the sample solution can flow through the solid phase extraction cartridge; the second aim is to clean the interference impurities and solvent residue on the SPE cartridge.
Solid-phase extraction (SPE) is usually used for the pretreatment of liquid samples, extraction of semi-volatile or non-volatile compounds, or removal of impurities in the sample that interfere with the separation analysis, and can also be used for pre-dissolution of the treatment. Solid-phase samples into solvents. SPE techniques are excellent for the extraction, concentration, and purification of analytes.
The solid-phase extraction cartridge is rinsed with a suitable solvent prior to sample extraction to keep the adsorbent moist and to adsorb the target compound or interfere with the compound. Different modes of SPE cartridge activation are different from solvents.
1. Reverse phase SPE
A weakly polar or non-polar adsorbent for reversed-phase solid-phase extraction is usually rinsed with a water-soluble organic solvent such as methanol and then rinsed with water or a buffer solution. It can also be rinsed with a strong solvent (such as hexane) prior to rinsing with methanol to eliminate impurities adsorbed on the adsorbent and its interference with the target compound.