How to Install a Solid Phase Extraction (SPE) Device

Installing a Solid Phase Extraction (SPE) device requires careful handling to ensure proper assembly and functionality. Here is a step-by-step guide for installing an SPE device:


  • Workstation Preparation: Ensure that the workstation is clean and organized. Prepare all necessary tools and accessories (such as connection tubes and fittings).
  • Personal Protection: Wear appropriate personal protective equipment, such as gloves and safety goggles.

Inspect Equipment and Components

  • Check Packaging: Verify that all components of the SPE device (such as extraction cartridges, connection tubes, and vacuum pump) are present and undamaged.
  • Clean Components: If necessary, clean all components to remove any manufacturing residues.

Connect Extraction Cartridge and Device

Select Appropriate Cartridges:

  • Choose the appropriate SPE cartridges based on the experimental requirements.

Connect the cartridge:

  • Fix the extraction cartridges onto the cartridge rack of the device. Ensure that the connections are tight to prevent any leakage.
  • Use suitable connection tubes to connect the extraction cartridges to the sample inlet of the device.

Connect the Vacuum System

Connect the Vacuum Pump:

  • Use chemically resistant vacuum tubes to connect the vacuum pump to the vacuum interface of the SPE device.
  • Ensure all connections are tight and free of leaks.

Check the Vacuum Pump:

  • Ensure the vacuum pump is functioning properly. Start the vacuum pump and check for any air leaks at the connections.

Install Solvent and Waste Bottles

Solvent Bottles:

  • Fill the solvent bottles with the required elution solvent and connect them to the sample inlet of the SPE device.
  • Use appropriate tubing and fittings to ensure the solvent can flow smoothly into the extraction cartridges.

Waste Bottles:

  • Connect the waste bottles to the waste outlet of the SPE device to ensure proper collection of waste liquids.

Test the System

Check Connections:

  • Ensure all connections are tight and leak-free.

Pre-treat Extraction cartridges:

  • Pre-treat the extraction cartridges with appropriate solvents as per the cartridge instructions to remove any impurities and activate the cartridges.

Test Run:

  • Perform a test run using water or another test solution to check the system for any blockages or leaks.
  • Adjust the vacuum pump pressure to ensure the extraction process runs smoothly.

Cleaning and Maintenance After Use

  • Clean the Equipment: Use appropriate cleaning solvents to clean the extraction cartridges and system tubing to ensure no residues are left.
  • Maintenance: Regularly check and maintain the vacuum pump, connection tubes, and other components to ensure long-term stable operation of the system.

By following these steps, you can correctly install and use a solid phase extraction device. Ensure to perform proper checks and maintenance before and after each use to guarantee the reliability of the experiments and the longevity of the device.