://How to Choose the Appropriate SPE Cartridges

How to Choose the Appropriate SPE Cartridges

Two factors will be considered generally when choosing the SPE cartridges, which are the selection of adsorbents and the selection of cartridge specification. The proper adsorbents will separate the interfering materials and the target substances better and satisfy the retrieving demands at the same time. And there exists upper limits of the quality of compounds adsorbed by adsorbents. Once the quality of adsorbed compounds exceeds, the target substances will pass over the SPE  cartridge straightly instead of remaining in it.

The selection of adsorbents

Adsorbents should be selected according to the properties of target compounds and substrates. As to the examples, we can choose the adsorbents based on the principle that distinguishes target substances and the major interfering materials.

Specification Maximum sample size Minimum elution volume
50mg 2.5mg 125μL
100mg 5mg 250μL
150mg 7.5mg 375μL
200mg 10mg 500μL
500mg 25mg 1250μL
1000mg 50mg 2500μL
Substrates Target substances Major Interfering Materials SPE Cartridges
Soil, Sewage hydrophobic organic pollutants Humic substances C18, SLC
Fruits, vegetables, Chinese medicine, fruits syrup pesticide residues Carbohydrates, pigments, organic acids, phenols NH2、Carbon/NH2
Pesticide residues with weak polarity C18
Alkaline drugs PCX
Acidic drugs PAX
Grease Liposoluble vitamins, phospholipids, aflatoxins fat NH2、PSA

The selection of cartridge specification

After choosing the proper adsorbent, we should choose the proper SPE cartridges with the best specification according to the volume of samples. And if the quality of the compounds to be measured is beyond the limit, the target substance will not be remained effectively, and the result of the elution is not good. Therefore, it is necessary to choose the proper specification.

Specification Maximum sample size Minimum elution volume
50mg 2.5mg 125μL
100mg 5mg 250μL
150mg 7.5mg 375μL
200mg 10mg 500μL
500mg 25mg 1250μL
1000mg 50mg 2500μL
2019-04-03T01:35:12+00:00March 27th, 2019|