How to Choose a Solid Phase Extraction Cartridges
The solid phase extraction cartridge is a sample pretreatment device for extraction, separation, and concentration developed from a chromatography column. Mainly used in sample pretreatment of various foods, agricultural and livestock products, environmental samples, and target compounds in biological samples.
Method for selecting solid phase extraction cartridge
The key to choosing a solid phase extraction cartridge is the packing that determines the separation performance in addition to the required specifications. When selecting an extraction column, it is necessary to select a suitable filler according to the type of sample to be tested and its physicochemical properties. Solid phase extraction fillers are usually chromatographic adsorbents and can be roughly divided into three categories, namely silica gel, high polymer, and inorganic materials.
The first type is based on silica gel, such as C18 solid phase extraction cartridge. The silica gel is very polar and weakly acidic. It can be used in normal phase or reverse phase separation mode: during normal phase extraction. The polarity is weaker than that of silica gel, and the non-polarity is weaker than C18 or C8 in reverse phase extraction. Good extraction results for steroids are commonly used for the extraction of non-polar or weakly polar compounds or the removal of polar impurities. It is mainly used for blood samples, drugs and their metabolites in urine samples, desalting of peptides, enrichment of trace organic compounds in environmental samples, and organic acids in beverages.
The fillers of solid phase extraction cartridges are based on high polymer and inorganic materials.
The second type is based on a high polymer such as polystyrene-divinylbenzene. High-purity, the high-crosslinking styrene-divinylbenzene polymer for stationary phase-filled SPE extraction cartridges with high loading capacity, withstand extreme pH conditions and different solvents, excellent for polar compounds and retention ability. The polymer can be used as a general-purpose adsorbent for acidic, neutral, and basic compounds. It is usually used to retain hydrophobic compounds containing hydrophilic groups such as phenols, nitroaromatics, nitramines, and nitrates under reversed phase conditions.
The third category is based on inorganic materials such as Floris diatomaceous earth, alumina, graphitized carbon, and the like. Flory Silica is a magnesium oxide composite polar silica adsorbent. The matrix SPE extraction column is suitable for the adsorption of polar compounds from non-polar matrices such as polychlorinated biphenyls, polycyclic aromatic hydrocarbons, organic Chlorine pesticide residues, etc.; Graphitized carbon black (CARB) extraction cartridge, with graphitized carbon black as a filler, the extraction process is very rapid. Moreover, the adsorption capacity of the compound is more than double that of the silica gel. Due to the positive six-membered ring structure on the surface of the graphitized carbon black, it has a strong affinity for planar molecules and is very suitable for extraction and purification of many organic substances, especially suitable for separation. Or remove various substances such as pigments, sterols, phenols, and other substances in fruits and vegetables; alumina-based fillers are acid, alkali, and neutral, suitable for separation and extraction of acidic, basic and neutral solvents. Hawach has a high-quality Alumina SPE column for your choice.
