Honey Analysis SPE Cartridge (C18 and SCX Samples Available)

The solid-phase cartridge is made from medical-grade PP housing and the 100,000-grade clean room-packed sorbent, with a higher recovery rate. The application of the cartridge can be related to food, beverage, honey, environment, biological analysis, and medicine.

We special supply the C18 SPE Cartridge and SCX SPE cartridge to our customer who is a famous honey exporter in Argentina, they used our cartridge when their QC part of the honey analysis, pesticide residue determination in honey. Some other customers’ use of determination of Amantadine Residue in honey by Solid-phase Extraction method would try MCX and C18A SPE Cartridge in their application.

Solid Phase Extraction (SPE) is a common technique used for the extraction, purification, and concentration of analytes from complex sample matrices. SPE cartridges with specific sorbent materials, such as C18 (reversed phase) and SCX (strong cation exchange), are employed for honey analysis to isolate and purify various compounds. Here’s how SPE cartridges, particularly those with C18 and SCX sorbents, can be utilized in honey analysis:

1. Sample Preparation:

  • Honey is a complex mixture containing sugars, organic acids, phenolic compounds, and other components. SPE cartridges aid in the preparation of honey samples for analysis by selectively extracting and concentrating target analytes.

2. C18 SPE Cartridge:

  • Purpose: C18 (reversed-phase) SPE cartridges are effective for extracting nonpolar to moderately polar compounds from honey, including phenolic compounds, flavonoids, and other lipophilic substances.
  • Procedure:
    1. Conditioning: The C18 cartridge is conditioned with an organic solvent (e.g., methanol) and equilibrated with water or a buffer.
    2. Sample Loading: The honey sample is loaded onto the cartridge, allowing the nonpolar and moderately polar compounds to interact with the C18 sorbent.
    3. Washing: Polar matrix components are removed by washing the cartridge with a suitable solvent.
    4. Elution: Target analytes are eluted with an organic solvent, and the eluate is collected for further analysis.

3. SCX SPE Cartridge:

  • Purpose: SCX (strong cation exchange) SPE cartridges are suitable for selectively extracting and concentrating cationic species such as organic acids and amino acids from honey.
  • Procedure:
    1. Conditioning: The SCX cartridge is conditioned with a salt solution or an acidic buffer.
    2. Sample Loading: The honey sample, containing cationic species, is loaded onto the cartridge.
    3. Washing: Non-target compounds are washed away, leaving the cationic analytes bound to the SCX sorbent.
    4. Elution: The cationic analytes are then eluted using a more concentrated salt solution or a stronger acidic buffer.

4. Analyte Analysis:

  • The eluates obtained from both the C18 and SCX SPE cartridges can be further analyzed using various analytical techniques such as High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), or Mass Spectrometry (MS) to identify and quantify specific compounds in honey.

5. Applications of Honey Analysis:

  • Quality Control: SPE is employed in honey analysis for quality control purposes, ensuring that honey meets regulatory standards and is free from contaminants.
  • Flavor Profiling: The extraction and concentration of specific compounds using SPE contribute to flavor profiling and authenticity testing of honey.
  • Phenolic Compound Analysis: C18 SPE cartridges are particularly useful for extracting phenolic compounds, contributing to the analysis of honey’s antioxidant properties.
  • Trace Element Analysis: SPE can also be utilized for the extraction of trace elements from honey samples for subsequent analysis.

6. Preventing Matrix Interference:

  • The selective extraction provided by SPE helps minimize matrix interference, allowing for more accurate and reliable analysis of target analytes in honey.

7. Optimization and Validation:

  • The SPE procedure should be optimized for the specific analytes of interest in honey. Validation procedures ensure the accuracy and reliability of the analytical results.

8. Regulatory Compliance:

  • Honey analysis using SPE is often conducted to meet regulatory requirements, ensuring that honey products meet established quality and safety standards.

9. Environmental Considerations:

  • SPE offers the advantage of reducing the environmental impact by concentrating analytes of interest and minimizing the use of organic solvents in the analysis.


Solid Phase Extraction (SPE) cartridges with C18 and SCX sorbents play a crucial role in the analysis of honey by facilitating the extraction, purification, and concentration of specific compounds. The selective nature of these sorbents allows for targeted isolation of analytes, contributing to accurate and reliable honey analysis for various purposes, including quality control, flavor profiling, and regulatory compliance. The choice of SPE sorbent depends on the nature of the analytes and the specific goals of the analysis.

Related Product Information

SLSPE5006C18A(SPE Cartridge, C18A, 75μm,60Å, 500mg/6mL,30pcs/pk)
SLNY1345NG(Non-sterile Nylon Syringe Filters, 0.45(μm), 13(mm), GF prefilter, 100pcs/pk)
1000-5000μl pipette
SLSPE2006SCX (SPE Cartridge,SCX, 40-75μm,70Å, 200mg/6mL, 30pcs/pk)
Mechanical Bottle-Top Dispenser, 2.5-25mL
Advanced Plus Mechanical pipette, 20-200μl, Autoclavable

Any more questions, please contact our sales department at info@hawach.com