Detail and Instruction of SPE Cartridge

Solid phase extraction (SPE) cartridge is a kind of sample pretreatment device developed from the chromatography column for extraction, separation, and concentration. It is mainly used for the pretreatment of target compounds in various food, agricultural, and animal products, environmental samples, and biological samples.SPE technology has been widely used in many GB/T and industry analysis standards.

SPE technology is based on the theory of liquid-solid chromatography and adopts selective adsorption and elution to enrich, separate, and purify the samples. It is a physical extraction process including liquid phase and solid phase. It can also be approximated as a simple chromatographic process. The more common method is to make the liquid sample solution through the adsorbent, retain the measured substance, choose the appropriate strength of the solvent to wash away the impurities, and then use a small amount of solvent to quickly elute the measured substance, so as to achieve the purpose of rapid separation, purification, and concentration. It can also selectively absorb interfering impurities and allow the measured substances to flow out. Or at the same time adsorb impurities and the measured substance, and then use an appropriate solvent to selectively elute the measured substance.
C18A SPE Cartridges
Most common SPE cartridges are injection syringes made of polyethylene, which are equipped with two polypropylene or fiberglass stoppers filled with a certain amount of chromatographic adsorbent (filler). The upper end of the SPE cartridge is open, and the lower end is the outlet. The liquid is discharged from the outlet after it passes through the adsorbent. According to the space volume above the classical spe column packing, the SPE column can be divided into 1 mL, 3 mL, 6 mL, 10 mL, 15 mL, 20 mL, 30 mL, 60 mL, etc. There are also spe columns of special specifications produced according to specific application requirements. Viewed from the outside of the spe column, the classic SPE cartridges are similar to the syringe and are straight. Another spe column has a funnel at the top, which allows more samples to be loaded at one time during manual operation.

The sieve plate SPE cartridge is a new type of monolithic SPE cartridge. This product USES the secret technology exclusive process to C18 and C8 bonded silica gel packing for special treatment so that it has high mechanical strength and toughness, with no need to add a polyethylene porous baffle as support in the SPE cartridge. The product of the secret proprietary technology “monolithic SPE cartridge is the earliest monolithic SPE cartridge in the domestic and foreign markets.

Solid phase extraction column without sieve plate

Product advantages of SPE cartridges

1. The packing is stable and can be fixed well, without common problems such as loose packing.
2. The integrated design of the whole packing effectively avoids the “ditch effect”.
3. You can choose to pay with a particle size as low as 8-10, which greatly improves the efficiency of separation and the amount of carrying samples.
The capacity of the SPE column refers to the adsorption capacity of the SPE column filler. For the solid phase extraction column based on silica gel, its capacity is generally 1~5 mg/100 mg, that is, the column capacity is 1%~5% of the packing mass.

The capacity of the SPE column refers to the adsorption capacity of the SPE column filler. For the solid phase extraction column based on silica gel, its capacity is generally 1~5 mg/100 mg, that is, the column capacity is 1%~5% of the packing mass. The capacity of bonded silica gel ion exchange adsorbent filler is expressed in meq/g, that is, the capacity per gram of filler is X mg equivalent. The packing capacity is usually between 0.5 and 1.5 meq/g.

Column capacity must be considered when selecting the SPE column. Because the sample matrix we are dealing with is often complex, such as food, biological samples, and so on. In spe, the adsorbent will absorb impurities of the same nature while adsorbing the target compound. Therefore, when considering the column capacity should be the target compound plus the total amount of impurities that can be adsorbed should not exceed the column capacity. Otherwise, some of the target compounds may not be adsorbed during the sample loading process, resulting in a low recovery rate.

The use of SPE cartridge

The simplest spe can be done manually by attaching a syringe to the top of the SPE column and squeezing the liquid out of the column. In addition, positive or negative pressure spe devices can also be used for spe operations on bulk samples. With the development of science and technology and the increase in sample quantity, more and more analytical laboratories have begun to use automatic spe, especially multi-channel spe for batch sample processing.

Tips and Considerations

  1. Sorbent Selection: Choose the SPE cartridge with a sorbent that is appropriate for your analytes (e.g., C18 for nonpolar, ion exchange for charged compounds).
  2. Flow Rates: Optimize flow rates during sample loading, washing, and elution to ensure efficient extraction without channeling.
  3. Solvent Compatibility: Ensure that the solvents used are compatible with both the sorbent material and the subsequent analytical technique.
  4. Sample pH: Consider the pH of your sample, as it can influence analyte-sorbent interactions.

Hawach has different types of SPE cartridges for your choice. If you like our product, don’t hesitate to contact us. We will reply as soon as we can. Looking forward to your reply.