Characteristics and Application of HLB Reverse Phase Extraction Column

The HLB reverse phase extraction column is a synthetic ultra-low pressure fast reverse phase chromatography packing material, and its base frame is a hard polystyrene molecule with uniform size, which can be operated in medium pressure column chromatography system and high pressure liquid phase system. The filler properties and method of use are similar to C18, which can replace C18, specific surface area of ??600m2/g, pore size of 70A, white powder. It is suitable for industrial fine separation and purification in the fields of natural products, chemical drugs and antibiotics. At the same time, it has better stability and wide pH applicability than C18. It can be used for the extraction of sulfonylurea herbicides, and can also be used for extraction, enrichment and purification of other polar or non-polar compounds. Average particle size: 40-60 μm, average pore diameter: 80, specific surface: 600 m 2 /g.

Product advantages:

Easy to operate, excellent flow rate range under natural gravity, good reproducibility;
It can save the cost of instruments and consumables without using solid phase extraction cylinders and

vacuuming equipment;
There is no blank background interference at all;
High recovery rate, the recovery rate of 10~100ppm is within the best range of 95%~105%;
Large adsorption capacity, 3 to 10 times that of C18;
Product quality is stable, reproducible, relative standard deviation (RSD) <0.1%;
Not afraid of dry columns. Both the stemming and the non-drying are the same within the error range, and the

relative standard deviation (RSD) is <0.05%;
At the same quality level as Waters’ Oasis HLB;
The price/performance ratio of products is among the best in the world.

Application range:

Soil, water, body fluids (plasma/urine, etc.), food, etc.

Typical applications:

Extraction and purification of drugs and metabolites from body fluids (plasma, urine, etc.);
separation of peptides and oligonucleotides;
High-throughput biological macromolecule desalination;
Trace organic matter, environmental pollutants, endocrine disruptors;
Japan JPMHW official method: antibiotics in food (such as fluoroquinolone, teralamycin, cephalosporin,

chloramphenicol, etc.), pesticide residues (sulfonylurea herbicides);
NY 5029: sulfonamides and beta-lactam antibiotics;
Diazepam, estrogen, diethylstilbestrol, tetracycline, macrolides, nitroimidazole, acrylamide;
NY/T 761.3: Carbamate pesticides.