Characteristics and Application of HLB Reverse Phase Extraction Column

The HLB reverse phase extraction column is a synthetic ultra-low pressure fast reverse phase chromatography packing material, and its base frame is a hard polystyrene molecule with uniform size, which can be operated in medium pressure column chromatography system and high-pressure liquid phase system. The filler properties and method of use are similar to C18, which can replace C18 SPE cartridges, a specific surface area of 600m2/g, a pore size of 70A, and white powder. It is suitable for industrial fine separation and purification in the fields of natural products, chemical drugs, and antibiotics. At the same time, it has better stability and wide pH applicability than C18. It can be used for the extraction of sulfonylurea herbicides, and can also be used for extraction, enrichment, and purification of other polar or non-polar compounds. Average particle size: 40-60 μm, average pore diameter: 80, specific surface: 600 m 2 /g.

The filler of polymeric HLB SPE cartridges produced by a special copolymerization technology contains a certain proportion of the hydrophilic group and the hydrophobic group. Hydrophobic divinyl benzene structures retain nonpolar compounds and hydrophilic n-vinyl pyrrolidone structures retain compounds. The filler of polymeric HLB SPE cartridges has good water wettability and can adjust the hydrophilic-lipophilic balance by water phase, so as to obtain ideal selectivity. The SPE cartridges have a good recovery rate of acid, neutral and basic compounds, especially suitable for the treatment of complex matrices such as blood, urine, and food.

Product advantages

Easy to operate, excellent flow rate range under natural gravity, good reproducibility;
It can save the cost of instruments and consumables without using solid-phase extraction cylinders and Vacuuming equipment;
There is no blank background interference at all;
High recovery rate, the recovery rate of 10~100ppm is within the best range of 95%~105%;
Large adsorption capacity, 3 to 10 times that of C18;
Product quality is stable, reproducible, and relative standard deviation (RSD) <0.1%;
Not afraid of dry columns. Both the stemming and the non-drying are the same within the error range, and the relative standard deviation (RSD) is <0.05%;
At the same quality level as Waters’ Oasis HLB;
The price/performance ratio of products is among the best in the world.

The characteristics of polymeric HLB SPE cartridge

1. Universal packing, wide application range;
2. High water infiltration, not easy to penetrate;
3. High recovery rate and good realization;
4. Adsorption capacity and sample load were much higher than C18 bonded silica gel;
5. Tolerable to PH 1-14, compatible with most solvents.

Application range of polymeric HLB SPE cartridge

Soil, water, body fluids (plasma/urine, etc.), food, etc.

i. Detect residues of the illegal drugs (antidepressants, heroin) and drug in the blood;
ii. Detect residues of antibiotics (penicillin, nitrofuran, chloramphenicol), catecholamine and microcystin toxin in food;
iii. Detect traces of veterinary drugs (tetracycline, quinolones), pesticides and mycotoxins in dairy products.

Typical applications

Extraction and purification of drugs and metabolites from body fluids (plasma, urine, etc.);
Separation of peptides and oligonucleotides;
High-throughput biological macromolecule desalination;
Trace organic matter, environmental pollutants, endocrine disruptors;
Japan JPMHW official method: antibiotics in food (such as a fluoroquinolone, rapamycin, cephalosporin, chloramphenicol, etc.), pesticide residues (sulfonylurea herbicides);
NY 5029: sulfonamides and beta-lactam antibiotics;
Diazepam, estrogen, diethylstilbestrol, tetracycline, macrolides, nitroimidazole, acrylamide;
NY/T 761.3: Carbamate pesticides.

Besides the HLB reverse phase extraction cartridges, you also can choose Ion-Exchange Amino (NH2) SPE Cartridge, SCX SPE Cartridges, SAX SPE Cartridges, Normal Phase PR Grade Florisil SPE Cartridges and other SPE Cartridges using.