Application of Normal Phase Solid Phase Extraction

Normal phase solid phase extraction is a versatile and increasingly popular sample preparation technique. SPE uses a solid adsorbent to adsorb a target compound in a liquid sample, separates it from the matrix and interfering compound of the sample, and then elutes with an eluent or desorbs by heating to achieve separation and enrichment of the target compound. Because of its safety, high recovery rate, good reproducibility, easy operation, fast, wide application range, easy to achieve automatic operation.

Normal phase solid phase extraction is commonly used as a positive phase stationary phase such as alumina, silica gel, polyamide, diatomaceous earth, activated carbon, etc., and the polar functional group of the measured object and the polar functional group on the surface of the filler pass hydrogen bonding. The interaction between the π-π bond, the dipole-dipole, and the dipole-induced dipole phase retains the polar species dissolved in the non-polar medium, and the polar solvent is often used as the eluent. Shao B et al used a silica gel column to purify 17 sulfonamide residues in animal-derived foods. The lower limit was 0.01 ng/g to 1.0 ng/g, and the recovery rate was 52% to 120%. The silica SPE column, amino-propane, and cyanide-propane SPE columns were compared. The acetone and methanol mixture was used as the eluent. The sensitivity of the silica gel column was the highest. The determination of seven hormone drugs in muscle by Seo J et al. Estradiol, testosterone, progesterone, dexamethasone, diethylstilbestrol, and estradiol metabolites, these drugs are easily soluble in non-polar solvents, the sample is first purified on a C8 column, and then eluted with a mixture of n-hexane-ethyl acetate the drug was retained in a silica gel column, and then the amino column was eluted with ethyl acetate-methanol.

Normal phase solid-phase extraction (NP-SPE cartridge) is a sample preparation technique used in analytical chemistry to separate and purify compounds from complex mixtures based on their polarity. It is particularly useful for nonpolar to moderately polar compounds. Here are some common applications of normal-phase solid-phase extraction:

  1. Polar Compound Isolation: Normal phase SPE cartridge is used to isolate and purify polar compounds from mixtures containing a wide range of compounds. These polar compounds can include various analytes, such as natural products, pharmaceuticals, and organic acids.
  2. Natural Product Analysis: Many natural products, including plant extracts and essential oils, contain a mixture of compounds with different polarities. Normal phase SPE cartridge helps to separate these compounds, making them suitable for further analysis or purification.
  3. Environmental Analysis: Normal phase SPE cartridge is employed in environmental monitoring to extract and purify polar contaminants from water, soil, and sediment samples. This is critical for the analysis of pollutants like pesticides, herbicides, and phenolic compounds.
  4. Pharmaceutical Analysis: In pharmaceutical research and quality control, Normal phase SPE cartridge is used to separate and purify polar and semi-polar compounds from drug formulations, metabolites, or complex matrices.
  5. Food and Beverage Analysis: Normal phase SPE cartridge is applied in food and beverage analysis to isolate and concentrate polar and semi-polar compounds, such as vitamins, amino acids, and organic acids, from complex food samples.
  6. Biochemical Research: Normal phase SPE cartridge is used to purify and concentrate biomolecules like peptides, proteins, nucleic acids, and carbohydrates from biological samples. It aids in the removal of interfering substances before downstream analysis.
  7. Sample Cleanup: Normal phase SPE cartridge is employed as a sample cleanup technique in various analytical methods, including gas chromatography (GC) and liquid chromatography (LC), to remove polar interferences that may affect the accuracy of analytical results.
  8. Phytochemical Analysis: In the field of phytochemistry, Normal phase SPE cartridge is used to fractionate and concentrate polar phytochemicals like flavonoids, tannins, and glycosides from plant extracts.
  9. Clinical and Forensic Analysis: NP-SPE can be utilized to purify polar drugs, metabolites, and other compounds from biological fluids (e.g., blood, urine) or forensic samples.
  10. Wastewater Treatment Monitoring: Normal phase SPE cartridges can help in monitoring and analyzing polar contaminants and organic compounds in wastewater samples, contributing to environmental protection efforts.

In Normal phase SPE cartridge, a polar sorbent (stationary phase) is often used to retain polar and moderately polar compounds, while nonpolar compounds pass through the column or cartridge. The selectivity of the stationary phase can be adjusted by varying the solvent polarity and elution conditions, allowing for the effective separation and purification of a wide range of compounds. Proper method development and optimization are essential to ensure a successful Normal phase SPE cartridge and obtain reliable analytical results.

The recovery of the sample after purification by a solid phase extraction column with three different retention mechanisms was 68% to 106%, and the lower limit was 0.1 μg/kg. 0.4 μg/kg. Hawach offers you Normal Phase Alumina SPE Cartridges, Normal Phase CN SPE Cartridges, Normal Phase Diol SPE Cartridges, Normal Phase PR Grade Florisil SPE Cartridges, and Normal Phase Silica SPE Cartridges.