Application Advantages of SPE

SPE is a sample pretreatment technology developed in recent years. It is developed by the combination of liquid-solid extraction and column liquid chromatography, which is mainly used for sample separation, purification, and concentration. Compared with traditional liquid-liquid extraction methods, it can improve the recovery rate of analytes and the more effective separation of the analyte and the interference component reduce the sample pretreatment process, and the operation is simple, time-saving and labor-saving.

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A solid-phase extraction device is a physical extraction process involving a liquid phase and a solid phase. In the solid-phase extraction process, the adsorption of the solid relative analyte is greater than that of the sample mother liquor. When the sample passes through the solid-phase extraction cartridge, the analyte is adsorbed on the solid surface, and the other components pass through the column with the sample mother liquor, and finally the appropriate the solvent takes off the analyte. It is widely used in the fields of medicine, food, environmental protection, commodity inspection, pesticide residues, etc.

SPE is a widely used and popular sample preparation technology that uses a solid adsorbent to adsorb a target compound in a liquid sample, separate it from the matrix and interfering compound of the sample, and then elute or heat it with the eluent. It is more ideal than ordinary solvent extraction in several cases:

1.Pretreatment of organic matter in water.
It is basically oscillating extraction with an organic solvent which is incompatible with water, and the advantages are as follows:

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(1) The pretreatment process can be repeated quantitatively. Solvent oscillation operation generally only requires the degree of control time, but can not control the oscillation frequency, intensity and action. We know that each person’s oscillation action is different, and it is difficult to ensure that the action is always uniform for the same person. Therefore, the action of solvent extraction is not quantitative and cannot be repeated. For SPE, it is easier to maintain the uniformity and stability of the column and the elution rate. Therefore, the extraction process of the SPE can be repeated and quantifiable.

(2) On-site processing.
The analysis of organic matter in water has a bottleneck that has long plagued many. That is to say, organic matter can be relatively stable in the environment such as ponds and reservoirs, but once it enters the small environment of the sampling bottle, it will change rapidly, so many organic matter analysis methods of water require immediate analysis. If the SPE technology is introduced, it can be sampled at the site and pretreated, as it is small in size and easy to carry. The sampler brought back to the lab was an SPE column instead of a water sample.

(3) Reduction in the consumption of organic reagents.
When using SPE, it is only necessary to use an organic solvent at the time of elution, which is a dozen times more than the conventional liquid-liquid extraction. It has positive significance for the personal protection and environmental protection of the experimenter.

2.Pharmaceutical ingredient extraction of bulk biomaterials
This is a successful example of solid-phase extraction in practical applications. It mainly refers to the pretreatment work when testing blood samples and urine samples in hospitals. With the single sample and fixed composition, it is suitable for large-scale batch purification operation after determining the method.