Compared to traditional liquid-liquid extraction and protein precipitation methods, solid phase extraction has unparalleled advantages. Purification method and advantages and disadvantages of solid phase extraction:
LLE Advantage No special equipment and materials required Disadvantage 1. Operation is cumbersome and time-consuming 2. It takes a lot of organic solvents, resulting in high costs and environmental pollution. 3. It is difficult to extract highly water-soluble substances from water PPT advantage Simple operation, no special treatment required Disadvantage 1. Non-specific precipitation reflects the possibility that a small amount of analyte will be lost as a result of the co-precipitation of matrix proteins. 2. The purification effect is weak, and the detection sensitivity and reliability are low. SPE advantage 1. The best way to improve the detection sensitivity is by integrating sample enrichment and purification 2. Faster than liquid and liquid extraction, saving solvent 3. Automated batch processing 4. Reproducible Disadvantage Requires professionals to continue to develop methods.