Activation Process Of SPE Cartridge
Hawach solid phase extraction cartridges are designed for sample pretreatment to remove or minimize sample matrix and other interferences to “clean up” a sample prior to analysis. This procedure can also concentrate an analyte to achieve the desired sensitivity range of an analytical method. Compounds are isolated from complex mixtures by proper selection of a variety of sorbent chemistries.
It is mainly used for sample pretreatment of various foods, agricultural and livestock products, environmental samples and target compounds in biological samples. Solid phase extraction technology has been widely used in many national standards (GB/T) and industry analysis standards.
The simplest solid phase extraction can be done manually, by attaching a syringe to the upper end of the SPE cartridge and extruding the liquid from the extraction cartridge by squeezing the syringe. Alternatively, a batch sample may be subjected to a solid phase extraction operation using a positive pressure or negative pressure solid phase extraction apparatus.
With the development of technology and the increase in the number of samples, more and more analytical laboratories began to use automated solid phase extraction, especially multi-channel solid phase extraction to process batch samples.
Configuration of SPE Cartridge
The common solid-phase extraction cartridge is mainly used as a needle tube type device with polyethylene as a material, and two pieces of a plug piece with the material of polypropylene or glass fiber are arranged in the device, and a certain amount of chromatographic adsorbent (filler) is filled in the middle of the two plug sheets. The upper end of the solid-phase extraction column is open, the lower end is a liquid outlet, and the liquid is discharged from the outlet after passing through the adsorbent. The size of the solid-phase extraction column can be divided into 1 mL,3 mL,6 mL,10 mL,15 mL,20 mL,30 mL,60 mL, and the like based on the volume of space above the classical solid-phase extraction column filler.