About The Role Of SPE Cartridge In The SPE Process
Solid phase extraction (SPE) is the use of a solid adsorbent to adsorb a target compound in a liquid sample, separating it from the matrix and interfering compound of the sample, and then eluting with an eluent or desorbing it to achieve the purpose of separating and enriching the target compound.
Compared with the traditional liquid-liquid extraction method, the recovery rate of the analyte can be improved, the analyte can be separated from the interference component more effectively, and the sample pretreatment process can be reduced, which is easy to operate, time-saving and labor-saving.
SPE cartridge, also called SPE cartridge is a sample pretreatment device for extraction, separation and concentration developed from a chromatography column. It is mainly used for sample pretreatment of various foods, agricultural and livestock products, environmental samples and target compounds in biological samples.
The capacity of the SPE cartridge refers to the adsorption amount of the cartridge packing. For silica-based SPE cartridges, the capacity is generally 1~5 mg/100 mg. The capacity of the bonded silica ion exchange adsorbent packing is expressed in meq/g. The capacity of such packing is usually between 0.5 and 1.5 meq/g.
The SPE device consists of an SPE cartridge and accessories. The SPE cartridge consists of three parts, a column tube, a sintered pad and a packing . SPE accessories generally include vacuum systems, vacuum pumps, blow dryers, inert gas sources, large capacity samplers, and buffer bottles.